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KMID : 0359319950350010029
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Korean Journal of Veterinary Research
1995 Volume.35 No. 1 p.29 ~ p.37
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The immunohistochemical study on the morphological changes of oxytocin secreting neurons in the age - related rat hypothalamus
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Abstract
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This study was carried out to investigate the morphological changes of the oxytocin secreting neurons in age-related rat hypothalamus by means of immunohistochemistry. The experimental group was 20 aged female rats (Sprague-Dawley, 25∼30 months), and the control group was 10 adult female rats (10 months). All animals were perfused transcardially with 4% paraformaldehyde-lysine-periocdate(PLP), and serial transverse brain sections(30 pm) were prepared by means of cryotome, and were stained with rabbit anti-oxytocin antisera immtrrtohistochemically, using the free floating method and avidin-viotin peroxidase complex. The results were as followings.
1. The immunostained oxytocin secreting neurons were located at the paraventricular nucleus and supraoptic nucleus of age-related rat hypothalamus chiefly.
2. The numbers of oxytocin secreting neurons decreased at the paraventricular nucleus and supraoptic nucleus of age-related rat hypothalamus(p<0.01)
3. The oxytocin secreting neurons of age-related rat hypothalamus immunostained less than those of the adult rat hypothalamus, and the paraventricular nucleus immunostained greater than supraoptic nucleus in age-related rat.
4. The numbers and expansion of dendrite and axonal varicosities decreased in the age-related rat hypothalamus greatly.
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